Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Oxidative determination of hydroxyamphetamine in drops using flow injection analysis].

J Dolejsová1, R Karlícek, M Pospísilová

  • 1Katedra analytické chemie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové.

Ceska a Slovenska Farmacie : Casopis Ceske Farmaceuticke Spolecnosti a Slovenske Farmaceuticke Spolecnosti
|August 23, 2000
PubMed
Summary

A new spectrophotometric method accurately determines hydroxyamphetamine using MBTH and ceric ammonium nitrate. This efficient technique is suitable for analyzing pharmaceutical products like Pedrolon drops.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chelators as Antineuroblastomas Agents.

Physiological research·2023
Same author

Murine experimental models for studying the pathogenesis of coxsackieviruses.

Acta virologica·2020
Same author

Small molecule inhibitors of DNA-PK for tumor sensitization to anticancer therapy.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2017
Same author

Fiber-optic pH detection in small volumes of biosamples.

Analytical and bioanalytical chemistry·2010
Same author

Determination of rhodamine 123 by sequential injection technique for pharmacokinetic studies in the rat placenta.

Talanta·2008
Same author

[Pindolol determination in pharmaceuticals by the method of capillary isotachophoresis].

Ceska a Slovenska farmacie : casopis Ceske farmaceuticke spolecnosti a Slovenske farmaceuticke spolecnosti·2005

Area of Science:

  • Analytical Chemistry
  • Spectrophotometry
  • Chemical Analysis

Context:

  • Accurate quantification of pharmaceutical compounds is crucial for quality control.
  • Existing methods for hydroxyamphetamine determination may lack efficiency or sensitivity.
  • Flow injection analysis offers rapid and automated sample processing.

Purpose:

  • To develop and validate a novel flow injection-spectrophotometric method for hydroxyamphetamine determination.
  • To utilize the reaction between hydroxyamphetamine, 2-hydrazono-3-methylbenzothiazole (MBTH), and ceric ammonium nitrate (CeIV).
  • To assess the method's performance in terms of sensitivity, precision, and sample throughput.

Summary:

  • A flow injection-spectrophotometric method was established for hydroxyamphetamine quantification at 540 nm.

Related Experiment Videos

  • The method employs MBTH and ceric ammonium nitrate (CeIV) as reagents, achieving a linear range of 0.01-0.2 mmol.l-1.
  • Optimized conditions yielded a relative standard deviation of 0.78% and a sample throughput of 75 samples per hour.

    • Impact:

    • Provides a rapid and precise analytical tool for hydroxyamphetamine in pharmaceutical formulations.
    • Demonstrates the applicability of the MBTH-CeIV reaction in flow injection analysis for drug quality control.
    • Successfully applied to the determination of hydroxyamphetamine in mass-produced Pedrolon drops.