Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Potassium-depolarization-induced cytoplasmic [Ca2+] transient in freshly dissociated pyramidal neurones of the rat

Z Rusznák1, C Harasztosi, P R Stanfield

  • 1Department of Physiology, University of Debrecen, Medical and Health Science Centre, Medical School, Hungary.

Pflugers Archiv : European Journal of Physiology
|August 23, 2000
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of one-year tofacitinib therapy on bone metabolism in rheumatoid arthritis.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2021
Same author

Different rates of endocytic activity and vesicle transport from the apical and synaptic poles of the outer hair cell.

HNO·2019
Same author

Comparison of peripheral quantitative computed tomography forearm bone density versus DXA in rheumatoid arthritis patients and controls.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2016
Same author

[Systemic sclerosis and pregnancy. A review of the current literature].

Zeitschrift fur Rheumatologie·2013
Same author

Effect of tacrolimus on the excitatory synaptic transmission between the parallel fibers and pyramidal cells in the rat dorsal cochlear nucleus.

Transplantation proceedings·2010
Same author

Serum concentrations of 25-OH vitamin D in patients with systemic lupus erythematosus (SLE) are inversely related to disease activity: is it time to routinely supplement patients with SLE with vitamin D?

Annals of the rheumatic diseases·2010

Voltage-activated calcium (Ca2+) channels in rat dorsal cochlear nucleus neurons were studied. N-type Ca2+ channels are primarily responsible for depolarization-induced increases in intracellular Ca2+ concentration.

Area of Science:

  • Neuroscience
  • Cell Physiology
  • Auditory System Research

Background:

  • Voltage-activated Ca2+ currents are crucial for neuronal excitability and intracellular Ca2+ signaling.
  • Pyramidal neurons in the dorsal cochlear nucleus play a key role in auditory processing.
  • Understanding Ca2+ channel function is vital for deciphering neuronal communication in the auditory pathway.

Purpose of the Study:

  • To investigate the role of voltage-activated Ca2+ currents in generating cytoplasmic Ca2+ transients in rat dorsal cochlear nucleus pyramidal neurons.
  • To identify the specific types of high-voltage-activated (HVA) Ca2+ channels responsible for these Ca2+ transients.

Main Methods:

  • Acutely dissociated pyramidal neurons from the rat dorsal cochlear nucleus were utilized.
  • Potassium (K+)-induced depolarizations were employed to evoke increases in intracellular Ca2+ concentration ([Ca2+]i).

Related Experiment Videos

  • The Fura-2 fluorimetric technique was used to monitor [Ca2+]i changes, and specific HVA Ca2+ channel antagonists (nifedipine, omega-agatoxin IVA, omega-conotoxin GVIA) were applied.

    • Main Results:

    • Resting intracellular Ca2+ concentration ([Ca2+]i) in these neurons was measured at 17.2+/-0.5 nM.
    • Neurons possessed caffeine-sensitive Ca2+ stores that were initially empty but could be rapidly filled and emptied.
    • Analysis of antagonist effects revealed that N-type Ca2+ channels predominantly mediate depolarization-induced [Ca2+]i transients.

    Conclusions:

    • N-type Ca2+ channels are the primary mediators of depolarization-induced intracellular Ca2+ transients in pyramidal neurons of the rat dorsal cochlear nucleus.
    • This finding elucidates the specific contribution of different Ca2+ channel subtypes to neuronal signaling in the auditory system.
    • The study highlights the importance of N-type channels in regulating Ca2+ dynamics within these critical auditory neurons.