Data evaluation in high-performance liquid chromatography-diode-array detection-fluorescence detection by information theory
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Updated: Aug 8, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
1Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, Romania. david@chem.unibuc.ro
Abstract:
The concepts of information theory were applied to the high-performance liquid chromatography (HPLC) technique, with diode-array (DAD) and/or fluorescence (FLD) detections. The information amount for a complete analysis can be computed as a function of analytical parameters, such as the number of analytes, level of concentration, and standard deviation of determinations. By means of the proposed method, the information content of a qualitative and quantitative analysis accomplished by HPLC-DAD-FLD was estimated, and sensitivity was optimized taking into account a maximum information content, while the detection limit was estimated considering that at this level of concentration the information content approaches zero.
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