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Detection and investigation of Campylobacter jejuni by polymerase chain reaction-restriction fragment length
1Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, Sagamihara Kanagawa, Japan.
Microbios
|August 24, 2000
Abstract:
A molecular typing approach for Campylobacter jejuni with restriction fragment length polymorphism (RFLP) analysis of the flagellin gene flaA in C. jejuni, was generated and studied. Using polymerase chain reaction (PCR)-RFLP with the restriction endonuclease Mbo I, it was demonstrated that C. jejuni could be divided into four types. Genotypic analysis of C. jejuni by PCR-RFLP is a valuable technique for epidemiological typing.