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Transient kinetics and intermediates formed during the electron transfer reaction catalyzed by Candida albicans
1Department of Pharmaceutical Chemistry, University of California School of Pharmacy, San Francisco, California 94143-0446, USA.
Biochemistry
|August 24, 2000
Abstract:
The transient kinetics of the reaction of the estrogen binding protein (EBP1) from Candida albicans in which hydride is transferred from NADPH to trans-2-hexenal (HXL) in two half-reactions were analyzed using UV-visible spectrophotometric and fluorometric stopped-flow techniques. The simplest model of the first half-reaction involves four steps including very rapid, tight binding (K(d)