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Related Experiment Videos

Intense exercise causes decrease in expression of both endothelial NO synthase and tissue NOx level in hearts.

M Iemitsu1, T Miyauchi, S Maeda

  • 1Cardiovascular Division, Department of Internal Medicine, Institute of Clinical Medicine, Tsukuba, Ibaraki 305-0006, Japan.

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
|August 24, 2000
PubMed
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Intense exercise significantly reduces nitric oxide (NO) production in the heart by decreasing endothelial NO synthase (eNOS) expression and lowering tissue nitrite/nitrate levels. This may explain increased heart rate and contractility during strenuous activity.

Area of Science:

  • Cardiovascular Physiology
  • Exercise Science
  • Molecular Biology

Background:

  • Cardiac myocytes are known to produce nitric oxide (NO).
  • Nitric oxide plays a role in regulating cardiac function, including responses to adrenergic stimulation.
  • The impact of intense exercise on cardiac NO production is not well understood.

Purpose of the Study:

  • To investigate the effects of intense exercise on nitric oxide synthase (NOS) expression in the heart.
  • To measure the levels of nitrite/nitrate (NOx), stable end products of NO, in the heart after exercise.
  • To determine if intense exercise alters cardiac NO production.

Main Methods:

  • Rats underwent a 45-minute treadmill exercise session.
  • Control rats rested for 45 minutes.

Related Experiment Videos

  • Heart tissue was analyzed for endothelial NOS (eNOS) mRNA and protein levels using RT-PCR and Western blot.
  • Cardiac tissue NOx levels were measured.

    • Main Results:

    • Exercised rats showed significantly lower eNOS mRNA levels compared to controls.
    • Western blot analysis confirmed a downregulation of eNOS protein in the hearts of exercised rats.
    • Tissue NOx levels were significantly reduced in exercised rats post-exercise.

    Conclusions:

    • Intense exercise leads to a decrease in cardiac NO production.
    • This reduction is associated with lower eNOS expression and tissue NOx levels.
    • The diminished cardiac NO production during intense exercise may contribute to increased myocardial contractility and heart rate.