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Development of polymerase chain reaction-based assays for bacterial gene detection
1Infectious Diseases (111F), VA Medical Center and Department of Medicine, University of Minnesota, Minneapolis 55417, USA. johns007@tc.umn.edu
Journal of Microbiological Methods
|August 26, 2000
Abstract:
Polymerase chain reaction-based assays provide rapid, simple, and sensitive detection of bacterial genes, but are not without their drawbacks. This review summarizes the principal advantages and disadvantages of PCR-based bacterial gene detection, provides guidelines for the development and validation of new PCR assays, and describes potential pitfalls that may be encountered and how these can be avoided.