Related Experiment Videos
Successful storage of RBCs for 9 weeks in a new additive solution.
1Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA. John.Hess@AMEDD.Army.Mil
Transfusion
|August 29, 2000
Summary
Storing packed red blood cells (RBCs) in experimental additive solution EAS 61 for 9 weeks maintains satisfactory quality. This extended storage improves transfusion availability and donor program efficiency.
Area of Science:
- Transfusion Medicine
- Blood Preservation
- Red Blood Cell Storage
Background:
- Investigated the efficacy of an experimental additive solution (EAS 61) for storing packed red blood cells (RBCs).
- EAS 61 is an alkaline, hypotonic solution containing adenine, dextrose, mannitol, NaCl, and Na(2)HPO(4).
Purpose of the Study:
- To evaluate the storage quality of packed RBCs suspended in EAS 61 for up to 9 weeks.
- To determine the impact of extended storage on RBC viability and biochemical parameters.
Main Methods:
- Packed RBC units were prepared from healthy donors and stored in 200 mL of EAS 61 for 8 and 9 weeks.
- In vitro assays and 24-hour in vivo autologous recoveries using (51)Cr were performed.
Main Results:
- Mean RBC recoveries were 81% at 8 weeks and 77% at 9 weeks.
- After 9 weeks, RBC ATP levels were 81% of initial, hemolysis was 0.35%, supernatant potassium was 46 mEq/L, and the morphologic index was 94.1.
Conclusions:
- Packed RBCs can be satisfactorily stored for 9 weeks in 200 mL of EAS 61.
- Extended RBC storage can reduce outdating and enhance transfusion availability, particularly in remote areas.