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Packing columns for capillary electrochromatography.
L A Colón1, T D Maloney, A M Fermier
1Department of Chemistry, State University of New York at Buffalo, Natural Sciences Complex, 14260-3000, USA. lacolon@buffalo.edu
Journal of Chromatography. A
|August 29, 2000
Summary
This study reviews capillary column packing methods for capillary electrochromatography (CEC). While slurry pressure packing is common, electrokinetic packing offers simplicity and potentially higher separation efficiencies for CEC applications.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrochromatography (CEC) is gaining interest for its separation capabilities.
- Packed capillary columns are crucial for effective CEC performance.
Purpose of the Study:
- To present and discuss various methods for packing capillary columns used in CEC.
- To provide detailed instructions for fabricating packed columns for researchers new to the field.
Main Methods:
- Slurry pressure packing
- Packing with supercritical CO2
- Electrokinetic packing
- Packing using centripetal forces
- Packing by gravity
- Entrapment methods (sintering, sol-gel)
Main Results:
- Slurry pressure packing is the most common method.
- Higher separation efficiencies can be achieved with alternative packing approaches.
- Electrokinetic packing is identified as a simple technique for material delivery.
Conclusions:
- Various methods exist for packing capillary columns for CEC.
- While common methods like slurry pressure packing are accessible, other techniques may yield superior separation efficiencies.
- Successful packed column fabrication for CEC requires skill and experience, regardless of the chosen method.