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Comparison of aqueous and non-aqueous capillary electrochromatography for the separation of basic solutes
1Imperial College of Science, Technology and Medicine, Zeneca/SmithKline Beecham Centre for Analytical Sciences, Department of Chemistry, South Kensington, London, UK. n.w.smith@ic.ac.uk
Abstract:
The analysis of basic compounds by capillary electrochromatography (CEC) on silica-based materials using conventional HPLC stationary phases has failed to address the problem of severe peak tailing and non-reproducible chromatography. Several new generation stationary phases were evaluated using aqueous and non-aqueous mobile phases. The best results were obtained in the aqueous mode using Waters Symmetry Shield RP-8, a material in which the residual silanol groups were shielded by an octylcarbamate function. For comparison, experiments were carried out using unmodified silica.