Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High temperature and temperature programming in capillary electrochromatography.

N M Djordjevic1, F Fitzpatrick, F Houdiere

  • 1Novartis Pharma AG, Core Technology Area, Basel, Switzerland. nebojsa.djordjevic@pharma.novartis.com

Journal of Chromatography. A
|August 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The development and evolution of the Irish Hip Fracture Database: a quality care initiative 2013-2024.

Archives of osteoporosis·2026
Same author

Reducing blood culture contamination in the ED: impact of Kurin Lock® implementation.

Infection prevention in practice·2026
Same author

A decade of Clostridioides difficile infection: a constant challenge to maintain the status quo.

The Journal of hospital infection·2023
Same author

Screening medical students for SARS-CoV-2 to facilitate face-to-face clinical teaching and prevent onward spread to patients.

The Journal of hospital infection·2023
Same author

Spontaneous Escherichia coli Meningitis and Pyogenic Ventriculitis in an Adult Receiving Anti-Tumour Necrosis Factor Alpha Therapy.

Irish medical journal·2022
Same author

Antimicrobial overuse in COVID-19 - reasons to remain vigilant as we approach the winter 'twindemic'.

The Journal of hospital infection·2022
Same journal

Portable cold-assisted head-space solid-phase microextraction coupled with GC-MS/MS for sensitive determination of trace polychlorinated naphthalenes in water.

Journal of chromatography. A·2026
Same journal

Characterization of phosphorous impurities originating from the synthesis of Sarin.

Journal of chromatography. A·2026
Same journal

Extraction and chromatographic purification of purpurin: A scalable approach using modified dry column vacuum chromatography.

Journal of chromatography. A·2026
Same journal

Development and validation of a modified QuEChERS-LC-MS/MS method for multi-class pesticide residue analysis in soils from pesticide production sites.

Journal of chromatography. A·2026
Same journal

Acid-treated ZIF-67-derived porous carbon based QuEChERS coupled with LC-MS/MS for sensitive determination of multi-pesticide residues.

Journal of chromatography. A·2026
Same journal

Polybenzimidazole-based magnetic solid-phase extraction coupled with gas chromatography-mass spectrometry for the determination of triazine herbicides in environmental waters.

Journal of chromatography. A·2026
See all related articles

Temperature programming in capillary electrochromatography (CEC) significantly reduces analysis time by up to 50% without compromising separation quality. This method offers a practical alternative to challenging solvent programming techniques.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Solute partitioning and electrophoretic mobility in electrochromatography are temperature-sensitive.
  • Controlled temperature variations can optimize solute selectivity and reduce analysis times.

Purpose of the Study:

  • To demonstrate the feasibility of temperature programming in reversed-phase capillary electrochromatography (CEC).
  • To compare temperature-programmed separations with isothermal, isocratic, and isorheic separations.

Main Methods:

  • Utilized a reversed-phase CEC mode.
  • Implemented temperature programming, varying both column temperature and mobile phase flow rate during separation.
  • Compared results against isothermal, isocratic, and isorheic conditions.

Main Results:

Related Experiment Videos

  • Temperature programming achieved up to a 50% reduction in separation run time.
  • The quality of separation was maintained despite the reduced run time.
  • Combined effects of temperature and flow rate changes were crucial for efficiency gains.

Conclusions:

  • Temperature programming is a viable and effective technique for capillary electrochromatography.
  • It presents a practical alternative to solvent programming, overcoming associated technical challenges.
  • This approach enhances efficiency and speed in CEC analyses.