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Related Experiment Videos

Decrease in ribosomal density of Proteus mirabilis exposed to subinhibitory concentrations of ampicillin or

V Lorian, L D Sabath, M Simionescu

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
    |July 1, 1975
    PubMed
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    Antibiotics like penicillin reduce bacterial ribosome density before causing cell wall defects. This challenges the view that cell wall synthesis is the primary target, suggesting ribosomes may be affected first.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Cell Biology

    Background:

    • Current understanding posits that penicillins and cephalosporins primarily target bacterial murein (cell wall) synthesis.
    • Antibiotic-induced cellular damage is typically characterized by defects in cell wall integrity.

    Purpose of the Study:

    • To investigate the initial cellular targets of penicillin and cephalosporin antibiotics.
    • To determine if ribosomal density changes precede cell wall defects.

    Main Methods:

    • Microscopic examination of bacterial cell sections.
    • Quantitative analysis of ribosomal density in response to antibiotic treatment.

    Main Results:

    • Reduced ribosomal densities were observed at antibiotic concentrations lower than those causing visible cell wall defects.

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  • No gross cell wall defects were evident in cells exhibiting decreased ribosomal frequency.

    • Conclusions:

    • Antibiotic-induced reduction in ribosomal density may precede cell wall damage.
    • This finding challenges the established model of antibiotic action, suggesting a potential primary effect on bacterial ribosomes.