Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Act1, an NF-kappa B-activating protein.

X Li1, M Commane, H Nie

  • 1Departments of Immunology and Molecular Biology, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.

Proceedings of the National Academy of Sciences of the United States of America
|August 30, 2000
PubMed
Summary

Researchers identified Act1, a novel NF-kappaB activator, which also activates Jun kinase (JNK). This suggests Act1 is part of a complex regulating both NF-kappaB and JNK pathways, potentially interacting with an unknown IL-1 signaling component.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gut Microbiota and Intestinal Monodomination as a Predictor for Bacteremia in Allogeneic Hematopoietic Cell Transplant Recipients.

The Journal of infectious diseases·2026
Same author

[Antibacterial activity of the antifungal peptide Mt6 - 21DLeu derived from <i>Musca domestica</i> against <i>Acinetobacter baumannii</i> and the underlying mechanisms].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2025
Same author

[Effects of periodontitis on bone and tryptophan metabolism of gut microbiota in estrogen-deficient mice].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2024
Same author

[Progress on microneedle drug delivery systems for the treatment of corneal diseases].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2024
Same author

Analysis of the mechanism of Sophorae Flavescentis Radix in the treatment of intractable itching based on network pharmacology and molecular docking.

European review for medical and pharmacological sciences·2024
Same author

[Interpretation of the European Federation of Periodontology S3 level clinical practice guideline for treatment of stage Ⅳ periodontitis].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2022

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Immunology

Background:

  • Nuclear Factor-kappaB (NF-kappaB) is a key transcription factor regulating immune responses and inflammation.
  • Interleukin-1 (IL-1) signaling pathways are crucial for innate immunity and inflammatory processes.
  • Jun kinase (JNK) pathways are involved in cellular stress responses and apoptosis.

Purpose of the Study:

  • To identify and characterize novel activators of NF-kappaB signaling.
  • To investigate the role of Act1 in NF-kappaB and JNK activation.
  • To elucidate the interaction of Act1 within the IL-1 signaling pathway.

Main Methods:

  • Isolation of a cell line expressing a cDNA for Act1 using an NF-kappaB-dependent selectable marker.
  • Assays to determine Act1's association with and activation of IkappaB kinase (IKK).

Related Experiment Videos

  • Analysis of Act1's effect on NF-kappaB, activating transcription factor (ATF), and activator protein 1 (AP-1) activation via JNK.

    • Main Results:

    • A cell line containing Act1 cDNA was successfully isolated, identifying Act1 as an NF-kappaB activator.
    • Act1 was found to associate with and activate IKK, leading to NF-kappaB liberation.
    • Act1 also activated JNK, indicating a potential role in a multifunctional signaling complex.
    • Act1 failed to activate NF-kappaB in an IL-1-unresponsive mutant cell line, suggesting interaction with an unknown IL-1 signaling component.

    Conclusions:

    • Act1 is a novel activator of both NF-kappaB and JNK signaling pathways.
    • Act1 may function within a multifunctional complex regulating inflammatory and stress responses.
    • The findings suggest the existence of an uncharacterized component in the IL-1 signaling pathway that interacts with Act1.