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Matrix-assisted laser desorption/ionization time-of-flight analysis of Bacillus spores using a 2.94 microm infrared
V Ryzhov1, J L Bundy, C Fenselau
1Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Rapid Communications in Mass Spectrometry : RCM
|August 30, 2000
Abstract:
The performance of infrared (2.94 microm) and ultraviolet (337 nm) lasers were compared for analysis of purified spores of B. subtilis, B. cereus and B. globigii on a four-inch end-cap reflectron time-of-flight instrument. Infrared matrix-assisted laser desorption/ionization (IR-MALDI) mass spectra of these microorganisms displayed a larger number of biomarker peaks above m/z 4000, compared with UV-MALDI. Biomarker peaks were observed at higher m/z values with the IR laser.