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Immunohistochemical investigation of migration inhibitory factor-related protein (MRP)-14 expression in
1Department of Pathology, Shizuoka General Hospital, Kitaando, Shizuoka, Japan.
Abstract:
Migration inhibitory factor-related protein (MRP)-8 and -14 belong to the S-100 protein family and are associated with myeloid cell differentiation. MRP is also expressed in some epithelia. However, there are few reports for the investigation on carcinomas. Using the monoclonal antibody 60B8 against MRP-14, we carried out the immunohistochemical evalution of MRP-14 expression in 70 cases of hepatocellular carcinoma (HCC), and examined the relation to tumor differentiation and vascular invasion. Positively stained tumor cells were detected in 32 cases, all of which belonged to grade II (7/30) or grade III (25/25) of the Edmondson-Steiner classification. In particular, the grade III HCC showed a significantly greater positive reaction. Immunopositivity in the non-carcinomatous hepatocytes and bile duct epithelia was not observed. These findings suggested that malignant hepatocytes newly express MRP-14 and that the neo-expression in differentiated HCC is related to the tumor differentiation and shows higher correlation in the poorly differentiated carcinomas. Furthermore, the cholangiocellular carcinoma and metastatic adenocarcinoma as control materials also presented a more marked immunoreactivity for MRP-14 in the poorly differentiated carcinomas, in a similar manner with the findings of the HCC. Accordingly, MRP is considered to be frequently neo-expressed in poorly differentiated carcinomas. MRP-14 expression rate in the 48 HCC cases with vascular invasion was 56%, showing no significant difference compared with non-invasive tumors.
Insights
Migration inhibitory factor-related protein (MRP)-14 is newly expressed in hepatocellular carcinoma (HCC), particularly in poorly differentiated tumors. Its expression correlates with tumor grade but not vascular invasion in HCC.
Area of Science:
- Oncology
- Molecular Biology
- Immunohistochemistry
Background:
- Migration inhibitory factor-related protein (MRP)-8 and -14 are S-100 proteins involved in myeloid cell differentiation and expressed in some epithelia.
- Limited research exists on MRP expression in carcinomas, necessitating further investigation.
Purpose of the Study:
- To evaluate MRP-14 expression in hepatocellular carcinoma (HCC) using immunohistochemistry.
- To examine the relationship between MRP-14 expression, tumor differentiation, and vascular invasion in HCC.
Main Methods:
- Immunohistochemical evaluation of MRP-14 expression in 70 HCC cases using the monoclonal antibody 60B8.
- Correlation analysis of MRP-14 expression with Edmondson-Steiner tumor differentiation grades and vascular invasion status.
Main Results:
- MRP-14 was expressed in 32 out of 70 HCC cases, predominantly in grade II and grade III tumors.
- Significantly higher MRP-14 expression was observed in grade III HCC compared to grade II.
- No MRP-14 immunopositivity was detected in non-carcinomatous hepatocytes or bile duct epithelia.
- MRP-14 expression was also more pronounced in poorly differentiated cholangiocellular carcinoma and metastatic adenocarcinoma.
- MRP-14 expression rate in HCC with vascular invasion (56%) showed no significant difference from non-invasive tumors.
Conclusions:
- Malignant hepatocytes exhibit neo-expression of MRP-14.
- MRP-14 neo-expression in HCC correlates with tumor differentiation, being higher in poorly differentiated carcinomas.
- MRP-14 is frequently neo-expressed in poorly differentiated carcinomas, including HCC, cholangiocellular carcinoma, and metastatic adenocarcinoma.
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