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Related Experiment Videos

Human granulocyte and reticuloendothelial system function during intralipid infusion.

C Jarstrand, L Berghem, G Lahnborg

    JPEN. Journal of Parenteral and Enteral Nutrition
    |November 1, 1978
    PubMed
    Summary

    Intralipid infusion impairs granulocyte function, reducing bacterial killing capacity. Lipid particle uptake by granulocytes likely hinders bacterial engulfment and impacts cell membrane function, decreasing their effectiveness.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • The reticuloendothelial system (RES) plays a crucial role in host defense.
    • Intralipid, a common lipid emulsion, is used clinically.
    • Understanding Intralipid's impact on immune cell function is important for patient care.

    Purpose of the Study:

    • To investigate the effects of Intralipid infusion on the phagocytic and bactericidal functions of granulocytes.
    • To explore the mechanisms underlying any observed changes in granulocyte function.

    Main Methods:

    • Assessed RES phagocytic and catabolic functions using 125I-microaggregated human serum albumin.
    • Measured nitroblue tetrazolium (NBT) reduction in granulocytes.
    • Evaluated bactericidal capacity against E. coli.

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  • Utilized electron microscopy to visualize granulocyte-lipid interactions.
  • Conducted in vitro experiments with varying Intralipid concentrations.

    • Main Results:

    • Intralipid infusion did not affect RES phagocytic/catabolic functions.
    • NBT reduction and bactericidal capacity of granulocytes were significantly decreased during Intralipid infusion.
    • In vitro, Intralipid dose-dependently reduced NBT reduction and bactericidal capacity.
    • Electron microscopy confirmed granulocyte phagocytosis of Intralipid particles.
    • Impaired function was observed even without plasma, suggesting a direct effect on granulocytes.

    Conclusions:

    • Intralipid infusion impairs granulocyte bactericidal capacity.
    • Phagocytosis of Intralipid by granulocytes may physically impede bacterial engulfment.
    • Lipid interaction with the granulocyte cell membrane likely contributes to reduced NBT reduction and overall decreased function.