Related Experiment Videos
Mithramycin represses MDR1 gene expression in vitro, modulating multidrug resistance
M Tagashira1, T Kitagawa, S Isonishi
1Foods and Pharmaceuticals Research and Development Laboratory, Asahi Breweries Ltd., Ibaraki, Japan. motoyuki.tagashira@asahibeer.co.jp
Abstract:
The effect of an aureolic acid, mithramycin (MTM) on multidrug resistance (MDR) was investigated. At a concentration of 0.02--0.1 mg/ml (about 20--90 microM), MTM repressed MDR1 gene transcription of SBC-3/ADM, a MDR-phenotype subline derived from human small cell lung tumor. Under the same conditions, another aureolic acid, chromomycin A3, showed potent cytotoxicity. FACS analysis revealed that 5 microm MTM depleted the P-glycoprotein (Pgp) and lowered the efflux activity of SBC-3/ADM cells. Furthermore, MTM sensitized the cells against adriamycin. These results suggest that MTM would be a useful modulator of MDR induced by Pgp.