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Effect of three proteinaceous foreign materials on infected and subinfected wound models
Abstract:
A model was devised to determine the effects of several foreign bodies (microcrystalline collagen hemostat (MCCH), blood clot, catgut suture) on S. aureus wound contamination and wound infection. In a subinfected wound model, MCCH, when compared with the other foreign bodies, showed an increase in the number of wounds classified as "contaminated," but 84 percent of these wounds yielded bacterial counts below the level accepted for biologically significant wound infection (10 to the 5th power bacteria per gram tissue). The number of "infected" wounds was not significantly greater than the number found in the uninoculated control group. In the infected wound model, all groups of inoculated animals showed significant infections. The severity of the wound infection was unaffected by the presence of catgut suture, clotted blood, or MCCH. Higher levels were not seen for the MCCH animals than for the inoculated control animals. We did not see in these experimental wound studies with MCCH a significant aggravation of wound contamination or wound infection during the 48 hour period following inoculation with varying dose levels of S. aureus. Further studies would be necessary to determine the possible bacteriological response after 48 hours.
Insights
Microcrystalline collagen hemostat (MCCH) did not significantly increase S. aureus wound contamination or infection in a 48-hour model. Bacterial levels remained below significant infection thresholds in most MCCH-treated wounds.
Area of Science:
- Microbiology
- Wound Healing
- Biomaterials
Background:
- Foreign bodies can influence wound contamination and infection.
- Staphylococcus aureus (S. aureus) is a common pathogen in wound infections.
- Microcrystalline collagen hemostat (MCCH) is a hemostatic agent used in surgery.
Purpose of the Study:
- To evaluate the impact of MCCH, blood clots, and catgut sutures on S. aureus wound contamination and infection.
- To assess if MCCH exacerbates S. aureus wound contamination or infection in experimental models.
Main Methods:
- A wound contamination and infection model was used in animals.
- The effects of MCCH, blood clot, and catgut suture were compared.
- Bacterial counts were measured in wound tissues at 48 hours post-inoculation.
Main Results:
- MCCH showed a slight increase in "contaminated" wounds, but 84% had counts below significant infection levels.
- The number of "infected" wounds was not significantly higher in MCCH groups compared to controls.
- Wound infection severity was not affected by the presence of MCCH, blood clot, or catgut suture.
Conclusions:
- MCCH did not significantly aggravate S. aureus wound contamination or infection within 48 hours.
- Further research is needed to determine long-term bacteriological responses to MCCH.
- MCCH appears safe regarding short-term S. aureus wound infection risk in this model.