An NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b
Related Experiment Video
Updated: Oct 13, 2026

06:32
Assessing Respiratory Immune Responses to Haemophilus Influenzae
Published on: June 29, 2021
1Laboratory for Molecular Structure, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts, EN6 3QG, UK.
Abstract:
We describe the use of Nuclear Magnetic Resonance (NMR) spectroscopy to control the identity of purified bulk capsular polysaccharide [called poly(ribosylribitolphosphate) or PRP] from Haemophilus influenzae type b (Hib), and derivatised forms, used in the production of Hib polysaccharide-protein conjugate vaccines. We describe the approaches we have developed to validate this test.

