Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acidic pH inhibits non-MHC-restricted killer cell functions.

B Fischer1, B Müller, K G Fischer

  • 1Institut für Biophysik und Strahlenbiologie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 23, Freiburg i. Br., D-79104, Germany.

Clinical Immunology (Orlando, Fla.)
|August 31, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Minimizing dispersive distortions in carrier-envelope phase sweeping with glass wedges.

Optics letters·2011
Same author

A German multicenter, randomized phase III trial comparing irinotecan-carboplatin with etoposide-carboplatin as first-line therapy for extensive-disease small-cell lung cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2011
Same author

Fully automated immunoassay for quantitative determination of FXIII.

Hamostaseologie·2011
Same author

Prevention, diagnosis, therapy, and follow-up of lung cancer: interdisciplinary guideline of the German Respiratory Society and the German Cancer Society.

Pneumologie (Stuttgart, Germany)·2010
Same author

Unusual splitting behavior of the dispersion of surface polaritons in gratings of different symmetry, amplitude, and profile.

Applied optics·2010
Same author

Cephalometric appraisal of a class I (Angle) malocclusion in which four first premolars were extracted as part of the treatment plan.

American journal of orthodontics and oral surgery·2010

Solid tumors create an acidic environment that impairs immune cell function. This study shows acidic pH inhibits key cytotoxic mechanisms, hindering anti-tumor responses and immunotherapy effectiveness.

Area of Science:

  • Immunology
  • Cancer Biology
  • Cellular Physiology

Background:

  • Immunotherapy for advanced solid tumors has limited success.
  • Tumor microenvironments often exhibit acidic interstitial pH.
  • Acidic pH is implicated in tumor cell immune evasion.

Purpose of the Study:

  • Investigate the impact of acidic extracellular pH on immune effector cell functions.
  • Elucidate mechanisms by which acidic pH inhibits anti-tumor cytotoxicity.
  • Assess the effect of pH on various immune cell killing pathways.

Main Methods:

  • Utilized peripheral blood mononuclear cells (PBMC) and IL-2-activated LAK cells as effector cells.
  • Assessed cytotoxic activity against TNFalpha-sensitive/resistant and Fas-positive/negative tumor cells.

Related Experiment Videos

  • Manipulated extracellular pH and calcium levels to analyze killing pathways (perforin/granzymes, TNFalpha, Fas/FasL).

    • Main Results:

    • Declining extracellular pH significantly inhibited perforin/granzyme release, TNFalpha secretion, and Fas/FasL-mediated cytotoxicity.
    • Acidic pH also affected the secretion of IFNgamma, IL-10, and TGF-beta(1).
    • A pH of 5.8 nearly abolished effector cell function by impairing mitochondrial activity and intracellular pH regulation.

    Conclusions:

    • Tumor acidity severely compromises essential immune effector cell functions.
    • Acidic tumor microenvironments contribute to immunosuppression in vivo.
    • Targeting tumor acidity could enhance anti-tumor immunity and immunotherapy efficacy.