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Isolation of novel mouse genes differentially expressed in brain using cDNA microarray.

T Yoshikawa1, Y Nagasugi, T Azuma

  • 1Biological Technology Laboratory, Helix Research Institute, 1532-3 Yana, Kisarazu-shi, Chiba, 292-0812, Japan.

Biochemical and Biophysical Research Communications
|August 31, 2000
PubMed
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Researchers identified nine highly expressed genes in the adult mouse brain using cDNA microarray technology. Further analysis confirmed their predominant brain expression, revealing novel genes and known nervous system markers.

Area of Science:

  • Genomics
  • Neuroscience
  • Molecular Biology

Background:

  • Determining gene expression patterns is crucial in the post-genome sequencing era.
  • Microarray technology enables simultaneous analysis of thousands of gene expression profiles.
  • Understanding brain-specific gene expression is vital for neuroscience research.

Purpose of the Study:

  • To identify highly expressed genes in the adult mouse brain using a cDNA microarray.
  • To validate the tissue distribution of these identified genes.
  • To discover novel genes with high brain expression.

Main Methods:

  • Utilized a cDNA microarray containing 2304 mouse cDNAs.
  • Analyzed gene expression in adult mouse brain, kidney, liver, and skeletal muscle.

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  • Performed tissue distribution analysis using reverse transcription-coupled polymerase chain reaction (RT-PCR).
  • Conducted database searches (GenBank/EMBL) for gene identification and homology.

    • Main Results:

    • Identified nine genes with high expression in the adult mouse brain compared to other tissues.
    • RT-PCR confirmed predominant brain expression for all nine selected genes.
    • Five known brain-expressed genes (MBP, SC1, HiAT3, S100 protein-beta, SNAP25) were identified.
    • One gene showed similarity to a rat/human homologue, suggesting a mouse homologue.
    • Three novel genes highly expressed in the brain were discovered.

    Conclusions:

    • The developed cDNA microarray system is effective for identifying differentially expressed genes in the mouse brain.
    • This study identified both known and novel genes predominantly expressed in the brain.
    • The findings contribute to a better understanding of mouse brain gene expression profiles.