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Apoptosis in human term placenta. A morphological and gene expression study.

M P Thiet1, V Suwanvanichkij, K Hasselblatt

  • 1Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Mass., USA.

Gynecologic and Obstetric Investigation
|August 31, 2000
PubMed
Summary

Apoptosis, programmed cell death, naturally occurs in the human term placenta. This study confirmed apoptotic processes in placental villi from healthy mothers after vaginal birth or cesarean section.

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Area of Science:

  • Reproductive Biology
  • Cellular Biology
  • Gynecology

Background:

  • The human placenta is crucial for fetal development.
  • Understanding placental cell turnover is vital for reproductive health.

Purpose of the Study:

  • To investigate the presence and patterns of apoptosis in term human placentas.
  • To compare apoptotic markers in placentas from vaginal deliveries versus cesarean sections.

Main Methods:

  • Analysis of placental tissues from 20 healthy term deliveries (10 vaginal, 10 cesarean).
  • Deoxyribonucleic acid (DNA) laddering assays to detect DNA fragmentation.
  • In situ terminal deoxynucleotidyl transferase mediated biotinylated dUTP nick end labeling (TUNEL) staining for apoptotic nuclei.
  • Reverse-transcription polymerase chain reaction (RT-PCR) to assess gene expression of apoptosis-related markers.

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Main Results:

  • DNA laddering, indicative of apoptosis, was observed in all placental samples.
  • TUNEL staining confirmed the presence of apoptotic nuclei within placental villi.
  • Messenger RNA (mRNA) expression for testosterone-repressed prostate message 2 (TRPM2) and B-cell lymphoma/leukemia-2 (Bcl-2) was detected.

Conclusions:

  • Apoptosis is a normal physiological process in the human term placenta.
  • These findings contribute to the understanding of placental development and aging.