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Related Experiment Videos

Correlation between apoptotic figure counting and the TUNEL technique.

V Vagunda1, J Kalabis, M Vagundová

  • 1Department of Pathology, Masaryk Memorial Cancer Institute, Brno, Czech Republic. vagunda@mou.cz

Analytical and Quantitative Cytology and Histology
|August 31, 2000
PubMed
Summary

Histologic detection of apoptotic figures is a cost-effective and reproducible method for analyzing colorectal carcinomas and lymphomas compared to TUNEL detection. Both methods showed a high correlation, but figure counting is preferred for routine diagnostics.

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Area of Science:

  • Pathology
  • Oncology
  • Cell Biology

Background:

  • Apoptosis, or programmed cell death, is a critical process in cancer development and progression.
  • Accurate detection of apoptotic cells is essential for tumor characterization and prognosis.
  • Histologic methods for detecting apoptotic figures and molecular techniques like TUNEL are available.

Purpose of the Study:

  • To compare the efficacy of direct apoptotic figure counting versus terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) for detecting apoptotic cells.
  • To evaluate these methods in formalin-paraffin embedded tissues from colorectal carcinomas and lymphomas.

Main Methods:

  • Evaluated apoptotic cell percentages in 39 tumor samples (29 colorectal carcinomas, 10 lymphomas) using both direct apoptotic figure counting and TUNEL.

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  • Utilized the Lucia G image analysis system for cellularity assessment and apoptotic figure counting.
  • Assessed correlation between the two methods using Pearson's correlation coefficient.

    • Main Results:

    • Apoptotic cells detected were 0.81% +/- 0.5% by figure counting and 0.91% +/- 0.35% by TUNEL.
    • A statistically significant high correlation (r = .56, P < .001) was observed between the two methods.
    • TUNEL assay showed higher sensitivity but presented challenges in standardization compared to figure counting.

    Conclusions:

    • Apoptotic figure counting is recommended as the preferred method for routine histopathologic diagnosis.
    • This method is favored due to its cost-effectiveness, reproducibility, and ease of implementation in diagnostic workflows.
    • Both methods are valuable, but figure counting offers practical advantages for standard laboratory practice.