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The Chlorella hexose/H(+)-symporters.

W Tanner1

  • 1Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Germany.

International Review of Cytology
|August 31, 2000
PubMed
Summary

The Chlorella kessleri HUP1 protein facilitates hexose uptake via proton symport, accumulating sugars efficiently. Its 12 transmembrane helices and unique kinetics are key to this vital plant membrane transport process.

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Area of Science:

  • Plant Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • The inducible hexose uptake protein (HUP1) from Chlorella kessleri is a crucial plant membrane transporter.
  • Encoded by the HUP1 gene, it is extensively studied for its role in sugar accumulation.

Purpose of the Study:

  • To review the physiology, molecular biology, and biochemistry of the Chlorella kessleri HUP1 protein.
  • To elucidate the transport mechanism, kinetics, and structural features of this hexose transporter.

Main Methods:

  • Literature review of HUP1 protein studies.
  • Analysis of kinetic data and structural predictions.
  • Examination of functional expression in various organisms.

Main Results:

  • HUP1 mediates hexose uptake via proton symport, achieving up to 1500-fold accumulation.
  • Transport involves 1 ATP investment per hexose and is driven by a large delta Km (Kminside >> Kmoutside).
  • The HUP1 protein comprises 12 transmembrane helices, with specific helices interacting during sugar translocation.

Conclusions:

  • HUP1 is a highly efficient hexose transporter belonging to the MFS-family.
  • Its transport mechanism and proton coupling differ from E. coli lac permease, despite structural similarities.
  • Functional expression of HUP1 has been demonstrated across diverse model organisms.

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