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Lactococcus lactis as a cell factory for high-level diacetyl production
J Hugenholtz1, M Kleerebezem, M Starrenburg
1Wageningen Centre for Food Sciences and NIZO Food Research, 6710 BA Ede, The Netherlands. hugenhol@nizo.nl
Applied and Environmental Microbiology
|August 31, 2000
Abstract:
We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.