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Published on: May 28, 2012
Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples
W L Tsai1, C E Miller, E R Richter
1Richter International, Inc., Columbus, Ohio 43201, USA.
Applied and Environmental Microbiology
|August 31, 2000
Abstract:
Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coli O157:H7, at the level of 10(-1) CFU/g of sample in either the 25- or 375-g sample size.
