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Construction of a multihybrid display system: flagellar filaments carrying two foreign adhesive peptides
J Tanskanen1, T K Korhonen, B Westerlund-Wikström
1Division of General Microbiology, Department of Biosciences, FIN-00014 University of Helsinki, Finland.
Applied and Environmental Microbiology
|August 31, 2000
Abstract:
A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in Escherichia coli. The inserted peptides were the fibronectin-binding 115-mer D repeat region of Staphylococcus aureus and the 302-mer collagen-binding region of YadA of Yersinia enterocolitica. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an in trans-complemented DeltafliC E. coli strain showed that individual filaments consisted of both recombinant flagellins.