Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure and function of hexameric helicases.

S S Patel1, K M Picha

  • 1Department of Biochemistry, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA. patelss@UMDNJ.EDU

Annual Review of Biochemistry
|August 31, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole genome sequencing and characteristics of extended-spectrum beta-lactamase producing <i>Escherichia coli</i> isolated from poultry farms in Banaskantha, India.

Frontiers in microbiology·2022
Same author

Visuomotor control in mice and primates.

Neuroscience and biobehavioral reviews·2021
Same author

Technique to manage intraoperative cuff leak from damaged endotracheal tube pilot balloon.

International journal of oral and maxillofacial surgery·2021
Same author

Metagenomic of clinically diseased and healthy broiler affected with respiratory disease complex.

Data in brief·2018
Same author

Improving bone marrow biopsy quality through peer discussion and data comparisons: A single institution experience.

International journal of laboratory hematology·2018
Same author

Complete Genome Sequence of Brucella abortus SKN 13 Isolated from Placenta of Aborted Cattle in Gujarat, India.

Genome announcements·2016

Hexameric helicases are ring-shaped motor proteins essential for DNA replication and transcription. Research reveals their nucleotide binding cooperativity and proposes models for DNA unwinding mechanisms.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Helicases are NTPase motor proteins crucial for nucleic acid unwinding.
  • Hexameric helicases, often ring-shaped, are vital for DNA replication, recombination, and transcription.
  • Defects in hexameric helicases are linked to human genetic disorders like Bloom's syndrome.

Purpose of the Study:

  • To elucidate the biochemical properties, structures, and DNA/nucleotide interactions of hexameric helicases.
  • To investigate the mechanisms of nucleotide binding cooperativity and sequential NTPase catalysis.
  • To propose models for DNA unwinding mediated by hexameric helicases.

Main Methods:

  • Analysis of biochemical properties and interactions.
  • Determination of crystal structures of oligomeric helicases (e.g., T7 gp4, RepA).

Related Experiment Videos

  • Development of mechanistic models for translocation and unwinding.

    • Main Results:

    • Observed cooperativity in nucleotide binding across multiple hexameric helicases.
    • Identified sequential NTPase catalysis in T7 gp4 and E. coli rho helicases.
    • Crystal structures reveal subunit interface nucleotide binding, supporting cooperativity.

    Conclusions:

    • Sequential NTP hydrolysis facilitates unidirectional and processive translocation by hexameric helicases.
    • Proposed DNA exclusion models (wedge, torsional, helix-destabilizing) for helicase-mediated unwinding.
    • Structural and biochemical data provide insights into hexameric helicase function and mechanisms.