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Apoptosis - when the cells begin to dance.

E Rudolf1, J Peychl, J Novak

  • 1Department of Medical Biology and Genetics, Charles University Faculty of Medicine in Hradec Kralove, Simkova 870, 500 01 Hradec Kralove I, Czech Republic. rudolf@lfhk.cuni.cz

Frontiers in Bioscience : a Journal and Virtual Library
|September 1, 2000
PubMed
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This study visualizes Hep-2 cells undergoing apoptosis after treatment with Etoposide, a topoisomerase II inhibitor. The observed "dance of death" includes cell rounding and membrane blebbing, offering insights into cellular responses to DNA damage.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Pharmacology

Background:

  • Etoposide is a chemotherapeutic agent that inhibits topoisomerase II, an enzyme crucial for DNA replication and repair.
  • Understanding the cellular mechanisms of Etoposide-induced apoptosis is vital for cancer treatment optimization.
  • Hep-2 cells are a commonly used human cancer cell line for in vitro studies.

Purpose of the Study:

  • To document and analyze the in vitro cellular morphological changes of Hep-2 cells upon treatment with Etoposide.
  • To visualize the dynamic process of apoptosis, specifically membrane blebbing, induced by a topoisomerase II inhibitor.
  • To provide a time-lapse video record of Etoposide's effect on Hep-2 cell behavior.

Main Methods:

  • Hep-2 cells were cultured in vitro and treated with 10 µg/ml Etoposide.

Related Experiment Videos

  • Cellular behavior was recorded over 24 hours using a Mitsubishi video recorder (HS-S5600).
  • Time-lapse videography was employed to capture dynamic morphological changes.

    • Main Results:

    • Observed typical cell rounding, a hallmark of apoptosis.
    • Documented the formation of numerous pseudopodia during cell death.
    • Captured rapid, rhythmical contractions known as membrane blebbing, termed the "dance of death".

    Conclusions:

    • Etoposide induces characteristic apoptotic morphological changes in Hep-2 cells, including cell rounding and membrane blebbing.
    • The "dance of death" phenomenon, characterized by blebbing, is a visually striking indicator of Etoposide-induced cellular demise.
    • Time-lapse videography provides a valuable tool for studying the dynamic processes of drug-induced apoptosis in cancer cells.