Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Protein stability in extremophilic archaea.

R Scandurra¹, V Consalvi, R Chiaraluce

¹Dipartimento di Scienze Biochimiche "A.Rossi-Fanelli" Universita 'La Sapienza', P.le A.Moro 5 00185 Rome Italy. scandurra@caspur.it

Frontiers in Bioscience : a Journal and Virtual Library

|September 1, 2000

Summary

Extremophilic proteins, adapted to harsh environments, do not follow general stabilization rules. Their stability relies on the same weak interactions found in mesophilic proteins, balancing stability and flexibility.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The role of Zn ions in the interaction between SARS-CoV-2 orf7a protein and BST2/tetherin.

European physical journal plus·2023

Same author

Metal Ion Binding in Wild-Type and Mutated Frataxin: A Stability Study.

Frontiers in molecular biosciences·2022

Same author

Adverse reactions following transfusion of blood components, with a focus on some rare reactions: Reports to the International Haemovigilance Network Database (ISTARE) in 2012-2016.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2022

Same author

Post-donation information and haemovigilance reporting for COVID-19 in Greece: Information supporting the absence of SARS-CoV-2 possible transmission through blood components.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2020

Same author

The association between the patient and the physician genders and the likelihood of intensive care unit admission in hospital with restricted ICU bed capacity.

QJM : monthly journal of the Association of Physicians·2018

Same author

Infection control interventions affected by resource shortages: impact on the incidence of bacteremias caused by carbapenem-resistant pathogens.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2017

Area of Science:

Biochemistry
Microbiology
Structural Biology

Background:

Extremophilic microorganisms thrive in extreme environments (e.g., high/low temperatures, pH, salinity).
Their proteins are naturally selected for stability under these harsh conditions.
Understanding extremophilic protein stability is crucial for basic and applied research.

Purpose of the Study:

To investigate the determinants of stability in extremophilic proteins.
To identify general or specific strategies employed by these proteins for survival.

Main Methods:

Analysis of extremophilic protein structures and sequences.
Comparative studies with mesophilic proteins.
Investigating the role of weak interactions (electrostatic, hydrophobic) in protein stability.

Related Experiment Videos

Main Results:

No universal rules for protein stabilization were identified.
Each extremophilic protein utilizes unique strategies for adaptation.
Stability is achieved through common weak electrostatic and hydrophobic interactions.

Conclusions:

Extremophilic protein adaptation does not follow a single rule.
The same fundamental interactions govern stability in both extremophilic and mesophilic proteins.
These interactions maintain a balance between protein stability and flexibility.