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Related Experiment Videos

Assignment of 60 human ESTs in cattle.

P Laurent1, C Elduque, H Hayes

  • 1Laboratoire de Génétique biochimique et de Cytogénétique, INRA, Jouy-en-Josas 78352 Cedex, France. plaurent@biotec.jouy.inra.fr

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|September 1, 2000
PubMed
Summary

Researchers mapped bovine DNA using human Expressed Sequence Tags (ESTs). They assigned 60 ESTs to the bovine genome, with 46 matching expected chromosome locations, advancing cattle genome research.

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Area of Science:

  • Genomics
  • Comparative Genomics
  • Molecular Biology

Background:

  • Large-scale cDNA sequencing has generated thousands of Expressed Sequence Tags (ESTs) as part of human genome studies.
  • Généthon mapped 10,000 human ESTs and identified approximately 1000 EST primers capable of amplifying bovine DNA.

Purpose of the Study:

  • To assign Type I sequences to the bovine genome using human ESTs.
  • To validate the utility of human ESTs for bovine genome mapping.

Main Methods:

  • Analysis of 233 primer pairs from Généthon.
  • Utilizing a hamster-bovine somatic cell hybrid panel for sequence assignment.
  • Polymerase Chain Reaction (PCR) to amplify bovine genomic DNA.
  • Single-Strand Conformation Polymorphism (SSCP) analysis to differentiate bovine and hamster PCR products when sizes were identical.

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Main Results:

  • 109 out of 233 primer pairs yielded specific PCR products with bovine genomic DNA.
  • 50% of these products showed identical sizes in cattle and hamster, necessitating SSCP analysis.
  • A total of 60 ESTs were successfully assigned to the bovine genome.
  • 46 of the assigned ESTs were located on the bovine chromosomes predicted by heterologous chromosome painting data between cattle and human.

Conclusions:

  • Human ESTs are valuable tools for mapping the bovine genome.
  • The study successfully assigned 60 ESTs to the bovine genome, confirming their utility in comparative genomics.
  • The findings provide a foundation for further detailed bovine genome annotation and comparative studies.