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Updated: Jul 30, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Regulation of cell cycle and apoptosis by human immunodeficiency virus type 1 Vpr
T Fukumori1, H Akari, A Yoshida
1Department of Virology, The University of Tokushima School of Medicine, 770-8503, Tokushima, Japan.
Abstract:
Biological effects of HIV-1 Vpr on CD4(+) cells were studied by an infection system. High-titered HIV-1 stocks pseudotyped with vesicular stomatitis virus G protein were prepared and used to inoculate into CD4(+ )T cells at high multiplicity of infection. Both cell- and virion-associated Vpr were demonstrated to arrest the cell cycle at the G2/M phase, and to induce cell apoptosis. Of note, morphologically apoptotic cells were shown to be arrested at the G2/M stage. No appreciable effect of Vpr on the anti-Fas antibody-mediated apoptosis was observed in this system.
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