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Updated: Oct 13, 2026
Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component
Published on: September 1, 2023
Molecular studies of the intestinal mucosal barrier physiopathology using cocultures of epithelial and immune cells:
S Kernéis1, E Caliot, H Stubbe
1Laboratoire des interactions lympho-épitheliales, département de bactériologie et de mycologie, Institut Pasteur, 28, rue du Dr. Roux, 75015, Paris, France.
Peyer's patch lymphocytes cocultured with Caco-2 cells trigger the phenotypic conversion of enterocytes into cells that express morphological and functional M-cell properties. We report a technical update for setting up this model, which will enable the study of M-cell biology, the identification by biochemical approaches of molecules involved in the interaction of microorganisms with M cells, and the development of vectors that would efficiently target the mucosal immune system.
Peyer's patch lymphocytes cocultured with Caco-2 cells trigger the phenotypic conversion of enterocytes into cells that express morphological and functional M-cell properties. We report a technical update for setting up this model, which will enable the study of M-cell biology, the identification by biochemical approaches of molecules involved in the interaction of microorganisms with M cells, and the development of vectors that would efficiently target the mucosal immune system.