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Corneal epithelial preservation.

R A Thoft, J Friend, H Freedman

    Archives of Ophthalmology (Chicago, Ill. : 1960)
    |May 1, 1975
    PubMed
    Summary
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    Prompt cooling of donor eyes after death is crucial for preserving corneal epithelium viability. Delayed cold storage, even by an hour, depletes essential glucose levels, compromising epithelial integrity and donor tissue quality.

    Area of Science:

    • Ophthalmology
    • Tissue Engineering
    • Cell Biology

    Background:

    • Current corneal storage methods are generally adequate for endothelial cells.
    • Increased use of donor epithelium necessitates re-evaluating storage effects on this layer.
    • Epithelial and stromal integrity can be compromised in severe ocular conditions.

    Purpose of the Study:

    • To re-examine the effects of storage techniques on the corneal epithelial layer.
    • To determine the critical time interval between death and cooling for preserving epithelial integrity.
    • To assess metabolite levels in stored corneas to understand preservation mechanisms.

    Main Methods:

    • Measurement of human and rabbit metabolite levels in stored corneas.
    • Analysis of glucose levels in corneal stroma and aqueous humor.

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  • Comparison of metabolite levels under different cooling delay conditions.

    • Main Results:

    • Prompt cooling of the eye is essential for maintaining normal epithelial state.
    • Delaying cold storage by one hour post-mortem significantly reduces stromal and aqueous glucose levels.
    • Reduced glucose levels fall below the threshold required for epithelial integrity preservation.

    Conclusions:

    • The time interval between death and cooling is critical for donor corneal tissue preservation.
    • Prompt enucleation and cooling are vital for maintaining corneal epithelial viability.
    • Applying ice to maintain viability of the epithelium is recommended if prompt enucleation is not feasible.