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Shear-induced assembly of lambda-phage DNA.

C Haber1, D Wirtz

  • 1Department of Chemical Engineering, Johns Hopkins University, Baltimore, Maryland 21218 USA.

Biophysical Journal
|September 2, 2000
PubMed
Summary
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Uniform shear flow controls DNA assembly, creating longer DNA multimers. This method enhances DNA concatenation for gene-chip and recombinant DNA technologies.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Polymer Rheology

Background:

  • Recombinant DNA technology relies on DNA fragment assembly.
  • Controlling DNA molecule assembly is crucial for biological research.

Purpose of the Study:

  • To demonstrate shear flow's ability to induce and control DNA assembly.
  • To investigate shear-induced DNA assembly mechanisms and applications.

Main Methods:

  • Applying uniform shear flow to lambda-phage DNA molecules.
  • Analyzing DNA multimer formation at varying shear rates.
  • Investigating shear-induced exposure of cohesive DNA ends.
  • Enhancing DNA concatenation kinetics using ligase.

Main Results:

Related Experiment Videos

  • Uniform shear flow induces and controls the assembly of lambda-phage DNA.
  • Increasing shear rates lead to integral DNA multimers of higher molecular weight.
  • Shear-induced assembly is attributed to increased molecular contact and stretching.
  • The method enhances DNA concatenation kinetics and extent in the presence of ligase.

Conclusions:

  • Shear flow provides a novel method for controlled DNA assembly.
  • This technique has potential applications in gene-chip and recombinant DNA technologies.
  • Shear-induced DNA assembly offers new insights into associating polymer rheology.