Related Experiment Videos
CD14+CD16+ monocyte subpopulation in Kawasaki disease
K Katayama1, T Matsubara, M Fujiwara
1Department of Paediatrics, Yamaguchi University School of Medicine, Yamaguchi, Japan.
Clinical and Experimental Immunology
|September 6, 2000
Summary
Kawasaki disease (KD) involves increased CD14+CD16+ monocytes, crucial inflammatory cells. Higher levels correlate with disease severity, suggesting their role in regulating monocyte function during acute KD.
Area of Science:
- Immunology
- Pediatrics
- Rheumatology
Background:
- Kawasaki disease (KD) is an acute febrile illness in children, characterized by vasculitis.
- Monocyte/macrophage activation is central to acute KD pathogenesis.
- The CD14+CD16+ monocyte subset is increasingly recognized for its role in inflammation.
Purpose of the Study:
- To investigate the peripheral blood CD14+CD16+ monocyte subpopulation in KD patients.
- To analyze serum levels of IL-10 and IL-12 in relation to KD.
- To compare these findings with other inflammatory conditions.
Main Methods:
- Flow cytometry was used to quantify CD14+CD16+ monocytes in 28 KD patients.
- Sandwich ELISA measured serum IL-10 and IL-12 levels.
- Comparisons were made with patients suffering from bacterial infections, mononucleosis, and anaphylactoid purpura.
Main Results:
- A significant increase in CD14+CD16+ monocytes was observed during acute KD.
- This increase positively correlated with C-reactive protein levels.
- Elevated serum IL-10, but not IL-12, was found in acute KD patients.
- Increased CD14+CD16+ monocytes were primarily seen in severe bacterial infections among controls.
Conclusions:
- Increased peripheral blood CD14+CD16+ monocytes are associated with acute Kawasaki disease.
- These monocytes may be involved in the regulatory system of monocyte function during KD.
- Elevated IL-10 levels further support immune dysregulation in acute KD.