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Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...

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Updated: Jul 23, 2026

Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
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Published on: February 4, 2017

The quantification of technetium in generator-derived pertechnetate using ICP-MS.

D M Hill1, R K Barnes, H K Wong

  • 1Australian Nuclear Science and Technology Organisation Menai, NSW.

Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
|September 6, 2000
PubMed
Summary

This study introduces a new method for measuring technetium-99 to technetium-99m ratios in medical generators. The validated technique accurately quantifies low-activity eluates for regulatory compliance.

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Area of Science:

  • Radiochemistry
  • Analytical Chemistry
  • Nuclear Medicine

Background:

  • Chromatographic technetium-99m (99mTc) generators, crucial for medical imaging, are derived from molybdenum-99 (99Mo).
  • Accurate measurement of the 99Tc:99mTc ratio is vital for regulatory compliance and ensuring generator performance.
  • Existing analytical methods struggle to accurately quantify this ratio in low-activity generator eluates throughout their elution cycle.

Purpose of the Study:

  • To develop and validate a sensitive analytical method for measuring 99Tc:99mTc ratios in 99mTc generator eluates.
  • To address the limitations of previous methods in monitoring low-activity generators.
  • To provide a reliable method for regulatory assessment of generator eluates.

Main Methods:

  • Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) was utilized to measure total technetium concentrations.
  • The method achieved a detection limit of 200 pg mL−1 (200 parts per trillion) for technetium in generator eluates.
  • 99Tc:99mTc ratios were determined and compared with theoretically computed values.

Main Results:

  • The ICP-MS method demonstrated high sensitivity, capable of measuring technetium down to 200 pg mL−1.
  • Measured 99Tc:99mTc ratios showed excellent agreement with theoretically calculated values across various elution stages.
  • Ratios varied from 47:1 for initial elutions of generators with long ingrowth times to 1.4:1 for later elutions of generators with short ingrowth times.

Conclusions:

  • ICP-MS provides a robust and sensitive analytical technique for determining 99Tc:99mTc ratios in 99mTc generator eluates.
  • This method overcomes previous limitations in monitoring low-activity generators and meets regulatory requirements.
  • The validated method ensures accurate assessment of generator quality and performance for nuclear medicine applications.