Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High intraluminal fluid flow increases intestinal IgA output.

N M Croft1, A Ferguson

  • 1Gastrointestinal Laboratory, Faculty of Medicine, University of Edinburgh. Western General Hospital, UK.

Scandinavian Journal of Gastroenterology
|September 6, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Every child, every day, back to play: the PICUstars protocol - implementation of a nurse-led PICU liberation program.

BMC pediatrics·2022
Same author

Analysis of misses in imaging of head and neck pathology by attending neuroradiologists at a single tertiary academic medical centre.

Clinical radiology·2021
Same author

COVID-19-induced toxic epidermal necrolysis.

Clinical and experimental dermatology·2021
Same author

Randomized controlled trial of topical corticosteroid and home-based narrowband ultraviolet B for active and limited vitiligo: results of the HI-Light Vitiligo Trial.

The British journal of dermatology·2020
Same author

An economic evaluation of the randomized controlled trial of topical corticosteroid and home-based narrowband ultraviolet B for active and limited vitiligo (the HI-Light Vitiligo Trial).

The British journal of dermatology·2020
Same author

Convolutional Neural Network-Based Automated Segmentation of the Spinal Cord and Contusion Injury: Deep Learning Biomarker Correlates of Motor Impairment in Acute Spinal Cord Injury.

AJNR. American journal of neuroradiology·2019

High intestinal fluid flow significantly increases immunoglobulin A (IgA) and other protein output, not due to reduced absorption. This suggests a non-specific response to acute diarrhea.

Area of Science:

  • Gastroenterology
  • Immunology
  • Physiology

Background:

  • Stool immunoglobulin A (IgA) output is lower in normal or chronic diarrhea compared to high-output watery diarrhea.
  • Hypothesized that increased intestinal fluid flow elevates IgA output, independent of degradation or reabsorption.
  • Investigated the relationship between intestinal fluid flow and protein output in the gut.

Purpose of the Study:

  • To quantify intestinal IgA and protein output in relation to fluid flow.
  • To determine if reduced degradation or reabsorption contributes to higher IgA output during high fluid flow.
  • To understand the physiological response of intestinal proteins to varying fluid conditions.

Main Methods:

  • Measured daily intestinal outputs of IgA and other proteins in stool from 14 ileostomy volunteers.

Related Experiment Videos

  • Compared ileostomy effluent protein output with whole-gut lavage fluid (WGLF) output using a perfusion system.
  • Analyzed protein recovery percentages and correlated IgA recovery with ileostomy effluent water content.

    • Main Results:

    • Significantly higher protein output was observed in whole-gut lavage compared to ileostomy effluent (P=0.02 to 0.001).
    • Median IgA output in ileostomy effluent was 3.6 mg/kg/day versus 26 mg/kg/day in WGLF, indicating only 12.7% recovery in stool.
    • Similar low recovery rates were found for other proteins; higher IgA recovery correlated with >92% ileostomy effluent water content.

    Conclusions:

    • High intraluminal fluid flow directly increases intestinal output of IgA and other proteins.
    • This phenomenon is not caused by decreased degradation or reabsorption in the gastrointestinal tract.
    • Elevated protein output may represent a non-specific host response during the initial phase of acute diarrhea.