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Updated: Oct 13, 2026

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens
Published on: March 19, 2019
Molecular recognition specificity of Bacillus globigii spore antibodies
1Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202, USA.
Abstract:
Western blotting methods have been used to assess the specificity of polyclonal antibodies raised against Bacillus globigii spore and vegetative cell preparations. None of the antibodies studied were completely species-specific in their recognition of spore surface epitopes. One polyclonal serum recognized several spore surface epitopes and demonstrated limited cross-reaction with the spore surface of the near-neighbour species B. subtilis. A second polyclonal serum, raised against aged spore antigens, recognized damaged spore epitopes primarily. Both of these antibodies also cross-reacted with vegetative cell epitopes present in all four Bacillus species (B. globigii, B. subtilis, B. cereus and B. anthracis) studied.
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