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Activation of murine macrophage-like cells by granulocytin
Y Fujita1, K J Homma, S Natori
1Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Biochemical and Biophysical Research Communications
|September 7, 2000
Abstract:
Granulocytin, a C-type lectin from Sarcophaga peregrina (flesh fly), stimulated glucose consumption and cytokine production by the mouse macrophage-like cell line J774.1. When J774.1 cells were pretreated with tunicamycin, their granulocytin-dependent TNF-alpha production was greatly reduced. These results suggest that the stimulus of granulocytin is transmitted to J774.1 cells via the carbohydrate chain of granulocytin receptors located on their surface.