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Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
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Purification of prostate-specific antigen from serum by indirect immunosorption and elution with a hapten

Hoesel1, Peter, Lenz

  • 1Roche Diagnostics GmbH, Nonnenwaldstrasse 2, 82372 Penzberg, Germany. Technical University Munich, 85748 Garching, Germany. author for correspondence: fax 49-8856-603341, e-mail wolfgang.hoesel@roche.com.

Clinical Chemistry
|September 7, 2000
PubMed
Abstract

No abstract available in PubMed .

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Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
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Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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