Related Experiment Video

Updated: Oct 13, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
10:00

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

Rapid and sensitive closed-tube quantification of human interferon-gamma mRNA by reverse transcription-PCR utilizing

Uehara1, Nardone, Randall

  • 1Intergen Discovery Products, 202 Perry Pkwy., Gaithersburg, MD 20877. author for correspondence: fax 301-963-5017, e-mail h. uehara@intergenco.com.

Clinical Chemistry
|September 7, 2000
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...