Human myeloid zinc finger gene MZF produces multiple transcripts and encodes a SCAN box protein

The myeloid zinc finger gene 1 (MZF1) encodes a C(2)H(2) zinc finger transcription factor that regulates granulopoiesis and may have a regulatory role in cellular proliferation and oncogenesis. The MZF1 gene has been previously reported to be 3kb and without introns. However, at least three transcripts of approximately 3, 7.5, and 9kb are detected by MZF1-specific probes in northern blot analysis and the identity of the transcripts has not been addressed. We screened a K562 cDNA library and identified novel transcripts, MZF1B and MZF1C. The 2.9kb MZF1B cDNA encodes a putative 734 aa protein and MZF1C maintains an identical open reading frame with 320 nucleotides deleted in the 5'-untranslated region. The MZF1B/1C protein contains all but the first eight amino acids of MZF1. Thus, MZF protein isoforms share 100 aa, as well as the bipartite 13 zinc finger DNA binding domain. In addition, MZF1B/1C encodes a unique 257 aa MZF1B/C amino terminus containing a SCAN box, or leucine-rich domain, which has recently been demonstrated to facilitate protein interactions. Sequence analysis reveals that the MZF gene contains six exons and spans 11kb and may be the most telomeric gene on chromosome 19q13. Exons 1-6 produce MZF1B/C cDNA, whereas MZF1 cDNA initiates within intron 5 and continues through exon 6. The 7.5 and 9kb transcripts are incompletely processed and contain intron sequences. These studies are the first description of the complete human MZF gene and of the composition of the multiple transcripts that are detected by northern blot analysis.