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Rabbit extracellular superoxide dismutase: expression and effect on LDL oxidation.
M O Laukkanen1, P Lehtolainen, P Turunen
1A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O. Box 1627, FIN-70211, Kuopio, Finland.
Gene
|September 7, 2000
Summary
Extracellular superoxide dismutase (EC-SOD) is an antioxidative enzyme. This study suggests EC-SOD protects against atherosclerosis by reducing low-density lipoprotein (LDL) oxidation and degradation.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiovascular Research
Background:
- Extracellular superoxide dismutase (EC-SOD) is a key antioxidative enzyme.
- EC-SOD exhibits high mRNA expression in the kidney and arterial wall.
- Understanding EC-SOD's expression and function is crucial for studying related diseases.
Purpose of the Study:
- To investigate the expression and antioxidative function of EC-SOD.
- To explore the antiatherogenic potential of EC-SOD.
- To analyze the effect of EC-SOD on oxidized low-density lipoprotein (oxLDL).
Main Methods:
- Cloning of rabbit ec-sod cDNA and production of recombinant EC-SOD protein.
- In vitro studies assessing mRNA synthesis versus protein activity.
- Evaluating the impact of EC-SOD on LDL oxidation and degradation in RAW 264 macrophages.
Main Results:
- In vitro studies indicated post-transcriptional regulation of EC-SOD, as mRNA levels did not directly correlate with protein activity.
- Recombinant EC-SOD significantly reduced the degradation of LDL by RAW 264 macrophages (28-36%).
- EC-SOD decreased the electrophoretic mobility of LDL oxidized by endothelial cells.
Conclusions:
- EC-SOD plays a role in regulating LDL oxidation and degradation.
- EC-SOD demonstrates protective effects against atherosclerosis development.
- Post-transcriptional mechanisms may influence EC-SOD activity and function.