[Morphometric analysis of cell nuclei and regional lymph node status with patients suffering from laryngeal...

We analysed 61 patients suffering from squamous cell carcinoma of larynx, who were treated in the Department of Otolaryngology, Collegium Medicum, Jagiellonian University, Cracow. Seven parameters of the cell nuclei and their variance were studied with the aid of a computer-assisted system of image analysis. The slides were viewed with a high-resolution black and white camera (Mintron) connected to the computer with the frame graber card (512 x 512 pixels with 256 gray levels, Vist software) at x630 magnification with an Axiophot microscope. It was established that high values of nuclear area, perimeter, density, long or shot nuclear axis carry an increased risk of metastases to regional lymph nodes.