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Related Experiment Videos

Fluospheres for cardiovascular phenotyping genetically modified mice.

C Richer1, V Domergue, M Gervais

  • 1Département de Pharmacologie, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France.

Journal of Cardiovascular Pharmacology
|September 7, 2000
PubMed
Summary

This study validates fluorescent microspheres (FMs) for assessing cardiovascular phenotypes in genetically engineered mice. The FM technique accurately measures cardiac output and regional blood flow, proving reliable for mouse cardiovascular research.

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Area of Science:

  • Cardiovascular Physiology
  • Mouse Models
  • Biomedical Engineering

Background:

  • Assessing systemic and regional hemodynamics in genetically engineered mice non-radioactively remains a challenge.
  • Previous methods using radioactive microspheres have limitations.
  • Fluorescent microspheres (FMs) have shown promise in rat studies.

Purpose of the Study:

  • To evaluate the fluorescent microsphere (FM) reference sample method for assessing hemodynamic phenotypes in C57BL/6 and apolipoprotein E (ApoE)-deficient mice.
  • To establish the feasibility, accuracy, and reliability of this nonradioactive technique in mice.

Main Methods:

  • Fluorescent microspheres were injected into the left ventricle of instrumented, anesthetized mice.
  • Cardiac output and regional blood flow distribution were measured.

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  • Validation included studies on mixing, simultaneous injection of dual-labeled FMs, and FM trapping.

    • Main Results:

    • The FM technique yielded results comparable to radioactive microspheres in C57BL/6 mice for cardiac output and regional distribution (e.g., brain, heart, kidneys, spleen, stomach).
    • The method demonstrated reliability across different age-matched groups and accurately reflected hemodynamic effects of angiotensin II and isoprenaline.
    • ApoE-deficient mice showed significantly decreased coronary blood flow compared to age-matched C57BL/6 controls, with no other major hemodynamic differences.

    Conclusions:

    • The fluorescent microsphere (FM) technique is feasible, accurate, and reliable for characterizing the cardiovascular phenotype of genetically engineered mice.
    • This nonradioactive method provides a valuable tool for mouse cardiovascular research.
    • The study identified reduced coronary blood flow as a potential hemodynamic alteration in ApoE-deficient mice.