[Cloning and sequence of cDNA encoding ACC synthase specifically expressed in banana fruit]

A cDNA encoding ACC synthase in banana pulp was amplified by RT-PCR and cloned in E. coli. The 5' terminal region of the ACC synthase transcript was determined by using 5' RACE procedure. The results showed that the ACC synthase cDNA in banana pulp is 1752 bp in length including 74 bp of 5' untranslated region, 1461 bp of coding region which encodes a polypeptide of 486 amino acides and 217 bp of 3' untranslated region. The Northern blot analysis indicates that the ACC synthase mRNA is specifically transcripted in banana fruits.