Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

GLAST: gene expression regulation by phorbol esters.

M Espinoza-Rojo1, E López-Bayghen, A Ortega

  • 1Departamento de Genética y Biología Molecular, Cinvestav-IPN, México DF, México.

Neuroreport
|September 8, 2000
PubMed
Summary

This study investigated glutamate/aspartate transporter (GLAST) gene expression in chick cerebellum. Protein kinase C activation decreases GLAST mRNA transcription, while cAMP signaling causes a transient reduction, indicating distinct regulatory pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Swimming respirometry provides insights into the physiological energetics of juvenile Atlantic bluefin tuna Thunnus thynnus.

The Journal of experimental biology·2026
Same author

93 Zeta potential of equine sperm and its association with sperm quality.

Reproduction, fertility, and development·2022
Same author

Effect of antimony in soils of an Sb mine on the photosynthetic pigments and antioxidant system of Dittrichia viscosa leaves.

Environmental geochemistry and health·2020
Same author

Endothelial dysfunction is an early indicator of sepsis and neutrophil degranulation of septic shock in surgical patients.

BJS open·2020
Same author

New Natural Hosts of Tomato spotted wilt virus.

Plant disease·2019
Same author

Current Status and New Natural Hosts of Tomato yellow leaf curl virus (TYLCV) in Spain.

Plant disease·2019

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Bergmann glia cells in the cerebellum play crucial roles in neurotransmitter homeostasis.
  • The Na+-dependent high affinity glutamate/aspartate transporter (GLAST) is vital for clearing excitatory amino acids from the synaptic cleft.
  • Understanding GLAST gene expression regulation is essential for comprehending cerebellar function and dysfunction.

Purpose of the Study:

  • To investigate the regulatory mechanisms controlling GLAST gene expression in cultured chick Bergmann glia cells.
  • To determine the effects of distinct signaling pathways, specifically protein kinase C (PKC) and cyclic adenosine monophosphate (cAMP), on GLAST mRNA levels.

Main Methods:

  • Cloning and sequencing of a 679 bp fragment of chick GLAST cDNA.

Related Experiment Videos

  • Quantitative analysis of chick GLAST mRNA levels using specific PCR primers.
  • Treatment of cultured cells with phorbol 12-tetradecanoyl-13-acetate (TPA), a PKC activator, and assessment of mRNA half-life.
  • Activation of the cAMP signaling pathway and observation of its effect on GLAST mRNA levels.

    • Main Results:

    • Treatment with the PKC activator TPA led to a significant decrease in GLAST mRNA levels.
    • TPA treatment did not alter the mRNA half-life, suggesting transcriptional downregulation of GLAST.
    • Activation of the cAMP pathway resulted in a transient decrease in GLAST mRNA levels.
    • The effects of PKC and cAMP pathways on GLAST mRNA levels were distinct, highlighting differential regulation.

    Conclusions:

    • GLAST gene expression in Bergmann glia cells is subject to regulation by distinct intracellular signaling pathways.
    • Protein kinase C activation mediates transcriptional downregulation of GLAST.
    • The cAMP pathway also influences GLAST expression, albeit through a transient mechanism.
    • These findings provide insights into the complex molecular mechanisms governing glutamate transporter expression in the cerebellum.