Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Robust parametric and semi-parametric spot fitting for spot array images.

N Brändle1, H Y Chen, H Bischof

  • 1Vienna University of Technology, Pattern Recognition and Image Processing Group. nob@prip.tuwien.ac.at

Proceedings. International Conference on Intelligent Systems for Molecular Biology
|September 8, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A massively multi-scale approach to characterizing tissue architecture by synchrotron micro-CT applied to the human placenta.

Journal of the Royal Society, Interface·2021
Same author

Purification and Application of Genetically Encoded Potassium Ion Indicators for Quantification of Potassium Ion Concentrations within Biological Samples.

Current protocols in chemical biology·2019
Same author

Pneumologie (Stuttgart, Germany)·2016
Same author

[ENT surgery in anticoagulate patients].

Laryngo- rhino- otologie·2015
Same author

Absence of significant structural changes near the magnetic ordering temperature in small-ion rare earth perovskite RMnO3.

Journal of physics. Condensed matter : an Institute of Physics journal·2014
Same author

Abnormal innervation of narrowed, uterine arterioles in cornual ectopic pregnancy.

Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology·2014
Same journal

Proceedings of the 8th International Conference on Intelligent Systems for Molecular Biology (ISMB 2000). San Diego, California, USA. August 19-23, 2000.

Proceedings. International Conference on Intelligent Systems for Molecular Biology·2001
Same journal

Analysis of gene expression data with pathway scores.

Proceedings. International Conference on Intelligent Systems for Molecular Biology·2000
Same journal

Towards a complete map of the protein space based on a unified sequence and structure analysis of all known proteins.

Proceedings. International Conference on Intelligent Systems for Molecular Biology·2000
Same journal

Mining for putative regulatory elements in the yeast genome using gene expression data.

Proceedings. International Conference on Intelligent Systems for Molecular Biology·2000
Same journal

A multiple alignment algorithm for metabolic pathway analysis using enzyme hierarchy.

Proceedings. International Conference on Intelligent Systems for Molecular Biology·2000
Same journal

Sequence database search using jumping alignments.

Proceedings. International Conference on Intelligent Systems for Molecular Biology·2000
See all related articles

This study introduces robust methods for analyzing gene expression data, accurately measuring gene labels even with overlapping spots. Novel techniques improve gene expression analysis accuracy and reliability.

Area of Science:

  • Genomics and Bioinformatics
  • Computational Biology
  • Biotechnology

Background:

  • High-density spot arrays are crucial for gene expression experiments.
  • Accurate measurement of bound labels per array element is essential.
  • Overlapping spots in gene expression images pose significant analytical challenges.

Purpose of the Study:

  • To develop reliable methods for fitting models to spots in high-density spot array images.
  • To accurately measure the amount of label bound to each array element.
  • To address challenges posed by overlapping and irregularly shaped spots.

Main Methods:

  • Utilized robust M-estimators to model Gaussian-shaped spots, effectively handling overlaps.
  • Developed a novel, robust semi-parametric method for cases where parametric fitting fails.

Related Experiment Videos

  • Implemented automatic detection for parametric model failure.

    • Main Results:

    • The proposed methods demonstrate accurate fitting of parametric and semi-parametric models.
    • Robust M-estimators successfully managed highly overlapping spots.
    • The semi-parametric approach accurately handled diverse spot shapes.

    Conclusions:

    • The introduced techniques provide reliable and accurate analysis of gene expression data from spot arrays.
    • These methods enhance the precision of measuring gene expression levels, especially in complex datasets.
    • The study offers improved computational tools for genomic research.