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Related Experiment Videos

pS86, a new theta-replicating plasmid from Enterococcus faecalis.

M Martínez-Bueno1, E Valdivia, A Gálvez

  • 1Dpto. Microbiología, Facultad Ciencias, Univ. Granada c/Fuentenueva s/n, 18071-Granada, Spain.

Current Microbiology
|September 8, 2000
PubMed
Summary
This summary is machine-generated.

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The complete nucleotide sequence of the Enterococcus faecalis plasmid pS86 was determined. This cryptic plasmid contains six open reading frames (ORFs) and likely replicates via a theta-mode mechanism.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genomics

Background:

  • Enterococcus faecalis is an opportunistic pathogen.
  • Plasmids play a crucial role in bacterial adaptation and evolution.
  • Cryptic plasmids, lacking obvious functions, are common in bacteria.

Purpose of the Study:

  • To determine the complete nucleotide sequence of the pS86 plasmid from Enterococcus faecalis ssp. faecalis S-86.
  • To identify potential open reading frames (ORFs) and their encoded proteins.
  • To investigate the replication and mobilization mechanisms of the pS86 plasmid.

Main Methods:

  • Whole-genome sequencing to determine the complete nucleotide sequence of the pS86 plasmid (5149 bp).
  • Bioinformatic analysis to identify putative open reading frames (ORFs).

Related Experiment Videos

  • Sequence similarity searches to identify functional domains and related proteins.

    • Main Results:

    • The complete nucleotide sequence of the pS86 plasmid was determined.
    • Six putative ORFs encoding polypeptides ranging from 7.3 to 65.1 kDa were identified.
    • ORF1 encodes a replication initiation protein (Rep), and ORF4 encodes a putative mobilization protein (Mob/Pre).
    • The pS86 plasmid shares sequence similarities with theta-type replicons from other Gram-positive bacteria.

    Conclusions:

    • The pS86 plasmid possesses genes for replication and mobilization.
    • The plasmid likely utilizes a theta-mode replication mechanism.
    • Further functional studies are needed to elucidate the roles of the remaining ORFs.